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How to capture the the weak fluorescence microscope image
Date: 2012/12/27 11:01:36
How to capture the weak fluorescence microscope image , has been microscope manufacturers and the image acquisition device manufacturers endeavor been subject for improvement. There are more and more ways to improve this situation: 1) First, it should be selected and quality fluorescence microscope, and applies to the fluorescence of the different fluorescence spectra of the objective lens; 2) to clean the sample carrier (e.g., glass slides, cover slides, etc.), eliminate unnecessary miscellaneous fluorescence interference; 3) try to improve sample preparation; 4) Select the appropriate excitation source; 5) to select the high sensitivity and large dynamic range CCD, of course, may be EMCCD class ; on the basis of the above conditions, the adjustment of the digital camera can be appropriate to increase the gain, but careful thus the signal-to-noise ratio decreased, the method of filtering can also be used to try to eliminate the noise signals. In addition, you want to reduce the re
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